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KMID : 0380420120360020081
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Journal of Prventive Veterinary Medicine
2012 Volume.36 No. 2 p.81 ~ p.88
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PrPsc detection in affected tissues and blood of mice infected with murine-adapted bovine spongiform encephalopathy strain 301C
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Lee Yoon-Hee
Sohn Hyun-Joo
Kim Min-Jeong
Kim Hyo-Jin
Lee Won-Yong
Choi Young-Pyo
Tark Dong-Seob
Joo Yi-Seok
Kweon Chang-Hee
Cho In-Soo
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Abstract
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The sensitivities of PrPsc detection methods, western blotting (WB), immunohistochemistry (IHC) and protein mis- folding cyclic amplification (PMCA) techniques were compared from brains, spleens and blood of mice challenged with PrPsc of murine-adapted BSE strain 301C. PrPsc was detected in the spleen from 30 dpi by IHC and at 60 dpi by WB. At 30 dpi, disease-specific signals of PrPsc was observed in only two follicles of a single spleen. PrPsc was detected in spleen at 10 dpi with PMCA after 5 rounds of amplification. Clinical signs were obviously shown from 240 dpi, and coincided with first detection of PrPsc in brains by WB, IHC and PMCA after one round amplitication. In addition, PrPsc was also detected in blood at 60, 180 and 240 dpi with PMCA after 5 rounds of amplification. The FDC-Ml epitope, which appears in immature FDCs, and PrPsc were detected in follicles first at 30 dpi, whilst the FDC-M2 epitope of mature FDCs was detected at 60 dpi. More FDC-M2 epitope and PrP were detected in follicles as disease progressed. The CD2 1/35 epitope is expressed on both FDCs and germinal center B cells. The pattern of CD2 1/35 expressing cells was similar to but less dominant than that of FDCs.
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KEYWORD
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PrPsc, WB, IHC, PMCA, Follicular Dendritic Cells
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